Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Comparative studies on the toxicological, anti-inflammatory and analgesic properties of three sources of Xuedan in mice and their rapid identification by electronic tongue

Hongling Zhang¹, Yi Qin¹, Qinwan Huang¹ , Jilin Sun², Renchuan Yao² Hairong Zeng

¹Chengdu University of Traditional Chinese Medicine, Chengdu 611137; ²Sichuan Fuzheng Pharmaceutical Co. Ltd, Chengdu 610041, China.

For correspondence:-

Accepted: 10 January 2019 Published: 28 February 2019

Citation: Zhang H, Qin Y, Huang Q, Sun J, Yao R.Zeng H. Comparative studies on the toxicological, anti-inflammatory and analgesic properties of three sources of Xuedan in mice and their rapid identification by electronic tongue. Trop J Pharm Res 2019; 18(2):257-262 doi: 10.4314/tjpr.v18i2.6

© 2019 The authors.
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Abstract

Purpose: To compare the toxicological, anti-inflammatory and analgesic properties of three sources of Xuedan, viz, Hemsleya omeiensis (HO), Hemsleya giganth (HG) and Hemsleya dolichocarpa (HD) in mice, and to study their rapid identification based on electronic tongue (E-tongue).

Methods: After 7 days of administration, the median lethal doses (LD₅₀) of the three xuedan decoctions in mice were determined. In addition, the anti-inflammatory and analgesic effects of the three xuedans were evaluated in mice using xylene-induced ear edema and acetic acid-induced pain. Furthermore, E-tongue technology was used to identify HO, HG and HD. Principal component analysis (PCA) and discriminant factor analysis (DFA) were used to analyze the data acquired by E-tongue.

Results: The median lethal dose (LD₅₀) values of H. omeiensis, H. gigantha and H. dolichocarpa were 32.3, 17.4 and 13.7g/kg, respectively. Compared with normal control group, the anti-inflammatory effects of Xuedan were obvious in xylene-induced ear edema (p < 0.05), and pain sensation was significantly inhibited in acetic acid-induced writhing test (p < 0.05). Furthermore, E-tongue technology effectively identified HO, HG and HD.

Conclusion: H. omeiensis exhibits the highest LD₅₀ value and best analgesic effect among the three sources of xuedan. E-tongue technology is effective and rapid in identifying HO, HG and HD.

Keywords: Xuedan, Hemsleya omeiensis, Hemsleya gigantha, Hemsleya dolichocarpa, Anti-inflammation, Analgesia, Electronic tongue